Tubing Connections

Masterflex Tubing Selection Guide: Complete Comparison

22 min read
Updated 11/9/2025

Complete comparison of Masterflex tubing materials including PharMed BPT, Tygon A-60 series, Norprene, C-Flex, and specialty formulations with detailed selection criteria and application recommendations.

Masterflex Tubing Selection Guide: Complete Comparison

Selecting the optimal tubing for your Masterflex peristaltic pump system is crucial for achieving reliable performance, extended service life, and chemical compatibility. This comprehensive guide compares all major Masterflex tubing formulations including PharMed BPT, Tygon A-60 series, Norprene, C-Flex, and specialty materials, providing detailed selection criteria and application recommendations.

Safety Precautions

⚠️ CHEMICAL COMPATIBILITY SAFETY

Always verify chemical compatibility between tubing material and process fluids before operation. Use manufacturer compatibility databases and conduct small-scale testing for critical applications. Incompatible combinations can result in tubing failure, contamination, or safety hazards. Wear appropriate PPE when handling chemicals and follow all OSHA guidelines for chemical safety in laboratory and industrial settings.

Tubing Selection Framework

Primary Selection Criteria

  1. Chemical Compatibility: Most critical factor
  2. Operating Temperature: Continuous and peak temperature requirements
  3. Pressure Requirements: System pressure and pressure pulsations
  4. Biocompatibility: USP Class VI, FDA, or other certifications required
  5. Service Life: Expected operating hours and replacement frequency
  6. Fluid Characteristics: Viscosity, abrasiveness, particle content
  7. Purity Requirements: Extractables, leachables, and contamination sensitivity
  8. Cost Considerations: Initial cost vs. total cost of ownership

Secondary Considerations

  • Clarity Requirements: Transparent vs. opaque applications
  • Sterilization Method: Autoclave, gamma, EtO compatibility
  • Regulatory Compliance: Industry-specific certifications
  • Environmental Factors: UV exposure, ozone resistance
  • Maintenance Schedule: Replacement frequency and procedures

Comprehensive Tubing Comparison

PharMed BPT (Biocompatible Pump Tubing)

Material Composition: Proprietary thermoplastic elastomer Primary Applications: Pharmaceutical, biotechnology, medical fluid handling

Key Specifications:

  • Biocompatibility: USP Class VI compliant
  • Temperature Range: -75°F to 275°F (-60°C to 135°C)
  • Pressure Rating: Varies by size, high-pressure variants available
  • Sterilization: Autoclave, gamma irradiation, EtO compatible
  • Service Life: Superior in chemically compatible applications

Performance Characteristics:

  • Chemical Resistance: Excellent for pharmaceuticals and biologics
  • Flexibility: Maintains flexibility at low temperatures
  • Recovery: Quick recovery from compression, ideal for viscous fluids
  • Extractables: Low extractables profile for sensitive applications
  • Particle Generation: Minimal spallation

Recommended Applications:

  • Cell culture media delivery
  • Pharmaceutical manufacturing
  • Bioprocessing and fermentation
  • Sterile solution transfer
  • High-purity water systems
  • Parenteral drug manufacturing

Tygon A-60-F (Food Grade)

Material Composition: Plasticized PVC formulation Primary Applications: Food, beverage, laboratory general use

Technical Specifications:

  • Certifications: FDA, 3A Sanitary, NSF-51
  • Temperature Range: -75°F to 275°F (-60°C to 135°C)
  • Pressure Rating: 19 PSI (1.37 bar)
  • Durometer: 61 Shore A
  • Sterilization: Autoclave up to 5 cycles
  • Color: Cream for easy fluid visibility

Size Examples:

  • I/P 26 (Catalog 0640226): 1/4" ID × 1/2" OD, 50 feet
  • I/P 88 (Catalog 0640288): Larger bore for higher flows

Performance Features:

  • Flexural Fatigue: Excellent resistance to repeated bending
  • Kink Resistance: Maintains flow under stress
  • Chemical Resistance: Acid/alkali resistant, compatible with sanitizers
  • Clarity: Translucent for visual flow monitoring
  • Cost: Economical choice for general applications

Recommended Applications:

  • Food and beverage processing
  • Laboratory fluid transfer
  • General chemical handling (compatible chemicals only)
  • Educational and training applications
  • Prototype and development work

Limitations:

  • NOT for medical or surgical use
  • Limited high-temperature continuous operation
  • Plasticizer migration in some solvents

Tygon A-60-G (Chemical Dispensing)

Material Composition: Advanced PVC compound with enhanced additives Primary Applications: Industrial cleaning, chemical dispensing, harsh environments

Technical Specifications:

  • Temperature Range: -75°F to 275°F (-60°C to 135°C)
  • Pressure Rating: 19 PSI (1.3 bar)
  • Durometer: 61 Shore A
  • Color: Black (for chemical identification and UV protection)
  • Bend Radius: 1/2 inch (for L/S 16 size)

Size Example:

  • L/S 16 (Catalog 0640416): 1/8" ID × 1/4" OD, 50 feet, $50.40

Advanced Properties:

  • UV Resistance: Superior resistance to UV degradation
  • Ozone Resistance: Excellent resistance to ozone exposure
  • Heat Resistance: Enhanced high-temperature performance
  • Chemical Resistance: Outstanding acid and base resistance
  • Heat Sealability: Can be heat-sealed for custom configurations

Recommended Applications:

  • Industrial cleaning systems
  • Chemical dispensing and dosing
  • Outdoor applications with UV exposure
  • High-temperature chemical handling
  • Acid/base titration systems
  • Environmental monitoring equipment

Advantages:

  • Exceptional environmental resistance
  • Long service life in harsh conditions
  • Cost-effective for industrial applications
  • Wide chemical compatibility range

Norprene Tubing

Material Composition: Thermoplastic elastomer with enhanced durability Primary Applications: High-pressure systems, extended-life applications

Performance Superiority:

  • Service Life: Best tubing life in chemically compatible applications
  • Pressure Handling: Optimal performance in pressure applications
  • Life Extension: 20-30% increased life with high-performance precision variants
  • Viscous Fluid Handling: Firm material provides quick recovery and accuracy

Available Configurations:

  • Standard Norprene: General-purpose applications
  • High-Pressure Norprene: Enhanced pressure capability
  • Precision Norprene: Tighter dimensional tolerances

Size Examples:

  • L/S 16 (Catalog 0640416): Standard precision size
  • L/S 15HP (Catalog 0650415): High-pressure rated, 25 feet

Technical Advantages:

  • Compression Recovery: Excellent elastic recovery prevents permanent set
  • Dimensional Stability: Maintains consistent internal diameter
  • Chemical Compatibility: Broad compatibility with laboratory chemicals
  • Temperature Stability: Consistent performance across temperature range

Recommended Applications:

  • High-pressure pumping systems
  • Long-duration continuous operation
  • Viscous fluid applications
  • Critical process applications where reliability is paramount
  • Systems with frequent pressure cycling

C-Flex Tubing Family

Material Base: Thermoplastic elastomer combining rubber flexibility with plastic durability

C-Flex ADCF (Standard)

Certifications: USP Class VI, manufactured under GMP Key Characteristics:

  • Tensile Strength: High tensile and tear strength
  • Gas Permeability: Low permeability for sensitive applications
  • Surface Quality: Smooth inner surface minimizes contamination
  • Clarity: Transparent for visual monitoring

Size Range:

  • Small Bore: 1/8" ID × 1/4" OD (Catalog 0643250), 50 feet
  • Medium Bore: 3/8" ID × 9/16" OD (Catalog 0643258), 50 feet

C-Flex ULTRA

Enhanced Properties:

  • Low Spallation: Reduced particle generation
  • Residue Reduction: Minimal buildup in pump heads
  • Biocompatibility: Enhanced biological compatibility
  • Clarity: Translucent for flow observation

Available Sizes: L/S 16, L/S 18 Catalog Numbers: 0643416, 0643418

C-Flex Opaque White

Special Features:

  • Light Blocking: Protects light-sensitive fluids
  • Size: 1/8" ID × 3/16" OD (Catalog 0642464), 25 feet
  • Applications: Photosensitive chemicals, biological samples

General C-Flex Advantages:

  • Chemical Resistance: Comparable to Tygon in most applications
  • Durability: Combines best properties of rubber and plastic
  • Contamination Resistance: Smooth surface reduces bacterial adhesion
  • Versatility: Suitable for wide range of applications

BioPharm Platinum-Cured Silicone

Material Technology: Platinum-cured silicone for enhanced purity Primary Applications: High-purity, pharmaceutical, biotechnology

Technical Specifications:

  • Biocompatibility: USP Class VI compliant
  • Certifications: USP Class VI, 3A sanitary standards
  • Curing Method: Platinum-cured (vs. peroxide-cured)
  • Temperature Range: -100°F to 400°F (-73°C to 204°C)
  • Surface: Ultra-smooth inner surface

Available Sizes: L/S Series: L/S 13, 14, 16, 24 (25 feet each) Catalog Numbers: 9642013, 9642014, 9642016, 9642024 I/P Series: I/P 73, 82 (25 feet each) Catalog Numbers: 9642073, 9642082

Premium Performance:

  • Service Life: BioPharm Plus lasts up to 5× longer than standard platinum-cured
  • Purity: Enhanced purity with reduced leaching
  • Sterilization: Compatible with all sterilization methods
  • Compression Set: Low compression set maintains sealing

Recommended Applications:

  • Pharmaceutical manufacturing (API and finished products)
  • Biotechnology processes
  • Cell culture and fermentation
  • Sterile solution transfer
  • Food processing (dairy, beverages)
  • High-purity water systems

GORE STA-PURE PCS-Series (Specialty)

Material Technology: Advanced fluoropolymer construction Target Application: Ultra-long-life I/P series applications

Exceptional Service Life:

  • At 200 RPM: Over 1000 hours
  • At 100 RPM: Over 6000 hours
  • Benefits: Eliminates frequent tubing rotation, saves maintenance time

Technical Features:

  • Spallation: Minimal particle generation
  • Flow Consistency: Maintains stable flow rates throughout life
  • Pressure Capability: Designed for I/P series pressure requirements

Available Sizes:

  • I/P 82: 1/2 inch ID (12.7 mm)
  • I/P 88: Large bore for maximum flow

Catalog Numbers: 9621182, 9621189, 9624182

Cost-Benefit Analysis: While premium-priced, the extended service life often provides lower total cost of ownership in continuous-operation applications.

Tubing Comparison Table

| Material | Biocompatibility | Max Temp (°C) | Max Pressure | Service Life | Chemical Resistance | Cost Level | |----------|-----------------|---------------|--------------|--------------|--------------------|-----------| | PharMed BPT | USP Class VI | 135 | Variable | Excellent | Pharmaceutical | Premium | | Tygon A-60-F | FDA Food | 135 | 19 PSI | Good | General Lab | Economy | | Tygon A-60-G | Industrial | 135 | 19 PSI | Very Good | Acids/Bases | Economy | | Norprene | General | Variable | High | Excellent | Broad | Standard | | C-Flex ADCF | USP Class VI | 80 | Moderate | Good | General | Standard | | C-Flex ULTRA | Enhanced | 80 | Moderate | Very Good | Enhanced | Premium | | BioPharm Silicone | USP Class VI | 204 | Moderate | Superior | High Purity | Premium | | GORE STA-PURE | Industrial | High | High | Ultra-Long | Specialized | Ultra-Premium |

Chemical Compatibility Guidelines

Compatibility Testing Protocol

  1. Literature Review: Check manufacturer compatibility databases
  2. Small-Scale Testing: Conduct compatibility tests with actual fluids
  3. Temperature Testing: Test at maximum operating temperature
  4. Extended Exposure: Long-term compatibility verification
  5. Performance Monitoring: Track any changes in properties

Common Compatibility Issues

Aggressive Chemicals:

  • Strong Oxidizers: May attack organic tubing materials
  • Aromatic Solvents: Can cause swelling in some formulations
  • Concentrated Acids: Require acid-resistant formulations
  • High-Temperature Applications: May accelerate chemical attack

Biological Fluids:

  • Protein Solutions: May require non-binding surfaces
  • Cell Culture Media: Biocompatibility and low extractables critical
  • Blood Products: USP Class VI certification typically required

Application-Specific Recommendations

Laboratory Applications

General Purpose: Tygon A-60-F

  • Cost-effective for non-critical applications
  • Good chemical resistance for common lab chemicals
  • Easy to replace and maintain

High-Precision Dispensing: BioPharm Plus Silicone

  • Excellent dimensional stability
  • Low compression set maintains accuracy
  • Ultra-smooth surface for consistent flow

Biological Applications: PharMed BPT

  • USP Class VI biocompatibility
  • Low extractables for cell culture
  • Sterilization compatibility

Industrial Applications

Chemical Processing: Tygon A-60-G or Norprene

  • Enhanced chemical resistance
  • Long service life reduces maintenance
  • Cost-effective for continuous operation

High-Pressure Systems: Norprene High-Pressure

  • Excellent pressure handling capability
  • Extended service life
  • Reliable performance under stress

Continuous Operation: GORE STA-PURE

  • Ultra-long service life
  • Minimal maintenance requirements
  • Consistent performance over time

Pharmaceutical Applications

API Manufacturing: PharMed BPT or BioPharm Plus

  • Regulatory compliance (USP Class VI)
  • Low extractables profile
  • Validated sterilization methods

Sterile Processing: BioPharm Plus Silicone

  • Steam sterilization compatible
  • Ultra-smooth interior surface
  • Minimal biofilm formation potential

Selection Decision Tree

Step 1: Regulatory Requirements

  • USP Class VI Required? → PharMed BPT, C-Flex ADCF, or BioPharm
  • FDA Food Contact? → Tygon A-60-F or BioPharm
  • No Special Requirements → Any compatible material

Step 2: Chemical Compatibility

  • Pharmaceutical/Biological → PharMed BPT or BioPharm
  • General Laboratory → Tygon A-60-F or C-Flex
  • Industrial Chemicals → Tygon A-60-G or Norprene
  • Aggressive Chemicals → Specialized materials or compatibility testing

Step 3: Performance Requirements

  • Maximum Service Life → GORE STA-PURE or Norprene
  • High Pressure → Norprene HP or specialized formulations
  • Ultra-High Purity → BioPharm Plus Silicone
  • Cost Optimization → Tygon A-60-F

Step 4: Operating Conditions

  • High Temperature → BioPharm Silicone
  • UV Exposure → Tygon A-60-G or GORE materials
  • Continuous Operation → Norprene or GORE STA-PURE
  • Intermittent Use → Any compatible material

Cost Analysis and Total Cost of Ownership

Initial Cost Comparison

Economy Tier: Tygon A-60-F, Tygon A-60-G

  • Lower initial cost
  • Suitable for non-critical applications
  • More frequent replacement may be needed

Standard Tier: Norprene, C-Flex ADCF

  • Balanced cost and performance
  • Good for most laboratory applications
  • Reasonable service life

Premium Tier: PharMed BPT, C-Flex ULTRA, BioPharm Plus

  • Higher initial cost
  • Superior performance characteristics
  • Often lower total cost of ownership

Ultra-Premium Tier: GORE STA-PURE

  • Highest initial cost
  • Exceptional service life
  • Lowest maintenance costs for continuous operation

Total Cost of Ownership Factors

  1. Tubing Cost: Initial purchase price
  2. Service Life: Hours of operation before replacement
  3. Labor Cost: Replacement time and technician rates
  4. Downtime Cost: Production loss during replacement
  5. Inventory Cost: Spare tubing storage and management
  6. Quality Cost: Impact of failures on product quality

ROI Calculation Example

Standard Application:

  • Tygon A-60-F: $50, 500-hour life = $0.10/hour
  • Norprene: $75, 1000-hour life = $0.075/hour
  • Labor + downtime savings with longer-life tubing often justify premium

Troubleshooting Tubing Selection Issues

Common Problems and Solutions

Short Tubing Life:

  • Check chemical compatibility
  • Verify pressure ratings
  • Consider premium formulations
  • Review operating conditions

Flow Rate Inconsistencies:

  • Evaluate tubing size selection
  • Check for chemical swelling
  • Consider dimensional stability of material
  • Verify proper installation

Contamination Issues:

  • Upgrade to higher-purity materials
  • Check extractables data
  • Consider surface smoothness
  • Evaluate cleaning/sterilization methods

High Replacement Costs:

  • Analyze total cost of ownership
  • Consider longer-life premium materials
  • Optimize replacement schedules
  • Implement preventive maintenance

External References

  1. Cole-Parmer Chemical Compatibility Database - Comprehensive compatibility information
  2. USP Class VI Testing Standards - Biocompatibility testing requirements
  3. Masterflex Technical Support - Professional assistance for material selection

⚠️ Legal Disclaimer: This guide provides general guidance for tubing selection based on manufacturer specifications and industry best practices. Always verify chemical compatibility through manufacturer resources and conduct application-specific testing. Material selection should be validated for each specific application considering all operating conditions and regulatory requirements.

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⚠️ Important Disclaimer

This guide summarizes safe repair practices based on industry best practices and manufacturer guidelines. Always consult the official manufacturer manual for model-specific procedures. Pump equipment repair should only be performed by qualified personnel following proper safety procedures and regulatory requirements.